OCT 29, 2020 6:00 AM PDT

Single-cell sequencing to investigate epigenetic mechanisms in inflammatory lymphocytes

Sponsored by: Illumina, Dolomite Bio
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Technical Applications Specialist, Dolomite Bio
    Biography
      Dr. Tom Ayers is a Technical Applications Specialist with Dolomite Bio. Tom completed his Doctorate in Molecular Biology from the University of Vienna in 2018. His research was focused on non-visual light detection and he identified a photoreceptor protein responsible for detecting sudden shadows in polychaete worms.
    • Sr Segment Marketing Manager EMEA, Cell Biology, Illumina
      Biography

        Pawel Zajac is the senior cell and molecular biology marketing manager in Europe, Middle East and Africa at Illumina. In this role, he is responsible for developing and executing the marketing plan for this segment. His prior roles at Illumina span several functions in sales and marketing. Pawel has a PhD from the Royal Institute of Technology (KTH) in Stockholm and worked as a PostDoc at the Karolinska Institute in Stockholm. His research focused on method development in single-cell RNA sequencing and multiplex amplification. Pawel is the author of >10 peer-reviewed articles in journals including Nature Methods and Genome Biology.

      • Group leader in systems biology, Botnar Research Centre, University of Oxford, United Kingdom
        Biography

          Dr Cribbs graduated with a PhD in molecular immunology from the Kennedy Institute of Rheumatology (Imperial college) in 2013. He then undertook a 2-year postdoctoral position within Prof Opperman’s laboratory at the University of Oxford. During his PhD and postdoctoral work Dr Cribbs’s work has focused on investigating the epigenetic pathways that control T cell inflammatory activation in autoimmunity.                                 Recognising the importance of computational biology to understand complex disease process in the functional genomics era, Dr Cribbs joined Prof Chris Ponting’s Computational Genomics and Analysis Training (CGAT) program as an MRC Training fellow at the University of Oxford. Here he developed a number of core competencies in statistics, mathematics and software development, enabling the difficult analysis and interpretation of next-generation sequencing data.                       Following the completion of his fellowship, Dr Cribbs joined the Botnar Research Centre in 2018 as a Group Leader in Systems Biology. Here he leads a computational biology team with a broad interest in systems biology, immunology and epigenetics. His work leverages computational and mathematical approaches to integrate different types of ‘omics’ data. This work facilitates the understanding the epigenetic pathways that regulate inflammatory function in the context of autoimmunity. In addition, outcomes from this work have facilitated the identification of candidate drug targets across multiple disease types, such as inflammation and oncology.


        Abstract
        Date:  October 29, 2020
        Time: 6:00am (PDT),  9:00am (EDT),  1:00pm (GMT),  2:00pm (CET)
         
        Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifications include DNA methylation and histone modifications and can be influenced by environmental exposure. Epigenetic changes are critical mediators of the functional changes that drive and maintain the inflammatory phenotype. Therefore, if we are to understand why the immune system is dysfunctional in chronic inflammatory conditions, we need to better understand the broad epigenetic pathways that regulate inflammation.
         
        In this webinar, Dr. Adam Cribbs from the Botnar Research Centre will describe the identification of three major epigenetic pathways (Histone deacetylases, Jumonji histone demethylases and BET bromodomains) that regulate inflammatory T cell and NK cell function in chronic inflammatory disease. Specifically, he will detail the work that led to the discovery of novel links between the role of Jumonji histone demethylases and the metabolism in Th17 cells. He will also share published and unpublished results showing how single-cell transcriptomics has been applied to better understand how these epigenetic pathways can be perturbed in order to identify therapeutic targets for chronic inflammatory disease and cancer.
         
        To conclude, Dr. Cribbs will present the challenges associated with applying single-cell technologies to understand the complex regulation of epigenetic pathways in inflammatory cells. Finally, he will share how these technologies can be applied to improve understanding of biological processes involved in immunity, facilitate drug development, and reduce health disparities.
         
        During this event Dr. Pawel Zajac from Illumina will also briefly introduce the latest sequencing news and Dr. Thomas Ayers from Dolomite Bio will present customizable single-cell workflows for varying cell types, sizes and applications enabled by the Dolomite Bio platforms.
         
        Join us to find out how single-cell sequencing can advance your research!
         
        Learning Objectives
        • Describe how to go from sample to insights with customizable single-cell sequencing workflows.
        • Recognize how leveraging chemical biology with functional genomic approaches provides insight into the epigenetic mechanisms regulating inflammation.
        • Identify and solve the challenges of applying single-cell technology to study epigenetic pathways in inflammatory cells.
         
         
        Webinars will be available for unlimited on-demand viewing after live event.
         
        LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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