Webinars

JAN 31, 2023 10:00 AM PST

Tips and tricks for successful multiplexing IHC

Sponsored by: Thermo Fisher Scientific

C.E. Credits: P.A.C.E. CE ｜ Florida CE

Speaker

Event Date & Time

Date: January 31, 2023

Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CEST)

Abstract

Immunohistochemistry (IHC) is a standard technique for scientists and pathologists to detect antigens. Examining one or two markers is a straightforward process but the level of complexity increases as more markers are added in complex samples (e.g., tumor microenvironments). Expanding to include more markers in an IHC experiment, requires in-depth knowledge about sample preparation, fluorescent dyes, and microscopes. This webinar will elucidate how to select markers and fluorophore dyes to a simple IHC experiment or start a larger Spatial Imaging panel. We will discuss how to prevent sample autofluorescence, unspecific antibody labeling, fluorescent bleed-through and spectral overlap.

Learning Objectives

Discover easy-to-incorporate techniques and tips to prevent nonspecific background effects or inadequate detection by dyes
Review basic methods from embedding, blocking, antibody labeling, mounting, and imaging the sample
Investigate free webtools to help design IHC experiments incorporating multiple fluorescent dyes

Webinars will be available for unlimited on-demand viewing after live event.

Labroots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

Tips and tricks for successful multiplexing IHC

Darpan Saraswat