NOV 18, 2020 7:00 AM PST

Troubleshooting Cell-based Assays - Ask the Experts to Avoid Common Pitfalls from Cell Seeding to Analysis

Sponsored by: Eppendorf, Promega
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Application Specialist Cell Handling, Eppendorf AG
    Biography
      Dr. Jessica Wagener is Application Specialist focusing on Cell Handling at Eppendorf Headquarter in Hamburg, Germany. Entering a cell culture laboratory as a graduate student for the first time in 2006, she came to Eppendorf with 5+ years of experience in cell culture. After studying Biology at the Universities of Marburg and Düsseldorf, she investigated apoptosis mechanisms in different cell lines for her diploma thesis. Following one year as Research Associate working with primary cells she started to work towards her PhD with a focus on reproductive biology at the Medical School Essen. She joined Eppendorf in April 2012 shortly after completing her PhD in Biology.
    • Application Specialist, Promega GmbH
      Biography
        Mr. Bonke received a Diploma in biology from the Johannes-Gutenberg University in Mainz, Germany in 2012. In his diploma thesis at the University Hospital in Mainz he worked at the genetic manipulation of murine embryonic stem cells in order to generate different transgenic mouse strains. He did his doctoral thesis at the University Hospital in Frankfurt am Main, where he focused on the mechanistic fundamentals of mitochondrial ROS generation and their physiological implication as cellular second messenger molecules, a process termed redox signaling. After completion of the experimental part of his thesis, Mr. Bonke joined Promega Germany to work as an Application Specialist with a main focus on cellular reporter technologies. As part of this position, he is giving frequent practical as well as theoretical workshops/seminars on the application of Promega's current luminescent reporter portfolio. In 2018, he was awarded a doctoral degree by the Johann Wolfgang Goethe University Frankfurt.

      Abstract
      DATE:  November 18, 2020
      TIME:  7:00am PST, 10:00am EST, 4:00pm CET
       
      The assessment of cell health and cellular responses after experimental manipulation continue to be a very important aspect of experimental biology. Reproducibility of cell-based assays is one of the key points when it comes to data reliability. Despite choosing the appropriate assay type there are many more factors to be considered to mitigate data variability. This joint Promega and Eppendorf webinar series gives a comprehensive overview of the cell culture workflow and introduce measures to maximize the outcome of your cell-based assay.
       
      In this episode, our experts will review the most frequently asked questions, that have been collected over the past years, including:
      • When to ideally time an analysis?
      • Does the passage number influence experimental outcomes?
      • When to choose what detection mode?
      • How to select the appropriate microtiter plate?
      • What is the best method for detecting mycoplasma?
      Join us to participate in the live Q&A session. If you can´t make it just register to receive the link to the recorded session.
       
       
      Webinars will be available for unlimited on-demand viewing after live event.
       
      LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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