SEP 23, 2021 9:00 AM PDT

Tubulin or Not Tubulin? Heading towards total protein staining as a loading control in immunological protein analytical methods

Sponsored by: ProteinSimple
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract
Date:  September 23, 2021
Time: 9:00am (PDT),  12:00pm (EDT)
 
Western blotting and other immunological protein-analytical methods are widely used for detecting and (semi-)quantifying specific proteins in given samples. This webinar will discuss a significant, but not yet well-established, improvement concerning the internal loading control as a prerequisite to accurately quantifying proteins by these methods. Currently, housekeeping proteins (HKPs) like actin, tubulin, or GAPDH are often used to check for equal loading or to compensate potential loading differences. However, these loading controls have multiple drawbacks. Staining of the total protein on the blotting membrane has emerged as a better loading control. Total protein staining (TPS) represents the actual loading amount more accurately than HKPs due to minor technical and biological variation. Further, the broad dynamic range of TPS solves the issue of HKPs that commonly fail to show loading differences above small loading amounts of 0.5–10 μg. Although these and further significant advantages have been demonstrated over the past 15 years, only a small percentage of laboratories take advantage of it. The objective of this webinar is to collect and compare information about TPS options and to invite users to reconsider their applied loading control. While the focus of this webinar will be on Western blots, similarities with other immunological protein-analytical methods will be illustrated and Simple Western total protein assay will be discussed.
 
Learning Objectives
  • Identify the advantages total protein staining has over classical housekeeping proteins when used as a loading control in Western blots
  • Describe how technical and biological variation is reduced when using total protein staining
  • Discuss the various total protein assays possible on Simple Western automated western systems
 
 
Webinars will be available for unlimited on-demand viewing after live event.

Show Resources
You May Also Like
APR 26, 2022 7:00 AM PDT
C.E. CREDITS
APR 26, 2022 7:00 AM PDT
Date: April 19, 2022 Time: 7:00am (PDT), 10:00am (EDT), 4:00pm (CEST) High-content (HC) phenotypic profiling approaches are a powerful tool to study the effect of biological, genetic, and ch...
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
APR 28, 2022 8:00 AM PDT
APR 28, 2022 8:00 AM PDT
Date: April 28, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Human pluripotent stem cells (PSCs) and their derivatives hold great potentials in...
MAR 02, 2022 9:00 AM PST
C.E. CREDITS
MAR 02, 2022 9:00 AM PST
Date: March 02, 2022 Time: 9:00am (PST), 12:00pm (EST) Single cell RNA-seq is known to only capture a small fraction of the transcriptome of each cell. Often, this is due to inherent limitat...
MAR 16, 2022 8:00 AM PDT
C.E. CREDITS
MAR 16, 2022 8:00 AM PDT
Date: March 16, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CET) Handling of potent and/or hazardous substances is commonplace in sev.....
DEC 09, 2021 6:00 AM PST
C.E. CREDITS
DEC 09, 2021 6:00 AM PST
Date: December 9, 2021 Time: 6:00am (PDT), 9:00 am (EDT) As the number of biotherapeutic candidates in development is increasing, the requirements of analytical tools to understand the molec...
Loading Comments...
Show Resources