OCT 15, 2020 8:00 AM PDT

Zonal Rotor Ultracentrifugation: An Effective Tool for Nanoparticle Separations at Large Scale

Speakers
  • Professor of Colloid Chemistry
    Biography

      Alexander Wittemann has been a professor of colloid chemistry at the University of Konstanz, Germany, since 2011. He received his diploma degree (2000) and his doctorate in chemistry (2004) from the University of Karlsruhe (TH), Germany. He then joined the Department of Physical Chemistry at the University of Bayreuth, Germany. After postdoctoral research in polymer science at McGill University, Montreal, Canada, he returned to Bayreuth, where he received his habilitation in physical chemistry in 2011. He was awarded from the Universitätsverein Bayreuth and the Dr. Otto Röhm Gedächtnisstiftung (2008). Wittemann received the Richard-Zsigmondy-Scholarship from the German Colloid Society in 2009. His current research focuses on complex nanoparticles, supracolloidal assembly and nanoparticle fractionation based on centrifugal methods.

    • Senior Researcher of Colloid Chemistry
      Biography

        Claudia Simone Plüisch is a Senior Researcher at the Chemistry Department of the University of Konstanz. In 2008, she was graduated in Polymer and Colloid Chemistry from the University of Bayreuth, Germany and received her doctorate (2012) with a thesis on supracolloidal polymer assemblies and their physicochemical investigation from the same institution. Her research interests are colloidal chemistry, materials science and nanoparticle assemblies. She has extensive expertise in the fractionation of nanomaterials by centrifugal techniques


      Abstract

      DATE:  October 15, 2020

      TIME:   8am PDT, 11am EDT

       

      Density gradient centrifugation is an effective method for the isolation and purification of small particles. Hollow rotors capable of hosting density gradients replace the need for centrifuge tubes and therefore allow separations at large scales. So far, zonal rotors have been used for biological separations ranging from the purification of whole cells down to serum proteins. We will demonstrate that the high-resolution separation method opens up exciting perspectives apart from biology, namely in sorting mixtures of synthetic nanoparticles. Loading and unloading, while the rotor is spinning, avoids perturbations during acceleration and deceleration periods, and thus makes a vital contribution to sorting accuracy. Nowadays one can synthesize nanoscale particles in a wide variety of compositions and shapes. A prominent example for this are “colloidal molecules” or, generally speaking, defined assemblies of nanoparticles that can appear in varying aggregation numbers. Fractionation of such multimodal colloids plays an essential role with regard to their organization into hierarchical organized superstructures such as films, mesocrystals and metamaterials. We will show that zonal rotor centrifugation is perfectly suited for getting “colloidal molecules” properly sorted.

       

      Learning Objectives:

      1. Insights into supracolloidal assembly.
      2. Understanding key principles of density gradient centrifugation.
      3. Performing nanoparticle separations using swing-out and zonal rotors.

       

       

      Webinars will be available for unlimited on-demand viewing after live event.

       

      LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.


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