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iQue® Human NK Cell Killing Kit | Sartorius

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The iQue ® Human NK Cell Killing Kit no wash assay, requires minimal hands on time and measures both cells and beads to provide the following biological insights: Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye. Target cells are distinguished from effector cells by staining with a fluorescent encoder dye. NK cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3- CD56+ NK cells. The panel also includes 2 different NK cell functional activation markers, CD69 and CD25. Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNγ and pro-apoptotic serine protease Granzyme B in the same assay well.

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